TNNI3 antibody (Middle Region)

Catalog No. ABIN2776782

This anti-TNNI3 antibody is a Rabbit Polyclonal antibody detecting TNNI3 in WB and IHC. Suitable for Human, Mouse, Rat, Dog, Cow, Rabbit, Goat, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for TNNI3 antibody (Middle Region) (ABIN2776782)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Goat, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TNNI3 Antibody

Binding Specificity: Middle Region

Sequence: RVRISADAMM QALLGARAKE SLDLRAHLKQ VKKEDTEKEN REVGDWRKNI

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against TNNI3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TNNI3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 210 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: TNNI3

Background: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. TNNI3 is the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues.Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CMH7, MGC116817, TNNC1, cTnI, RCM1, CMD2A, CMD1FF
Protein Interaction Partner: TNNC2, TRIM63, TRIM55, RCAN3, TNNC1, TNNI3K, PKD2L1, TNNI3, TNNT2, TNNT1, PRKCA, PAPPA, PKD2, ACTA1, LYST,
Protein Size: 210

Molecular Weight: 23 kDa

Gene ID: 7137

NCBI Accession: NM_000363, NP_000354

UniProt: Q59H18