ASL antibody (N-Term)

Catalog No. ABIN2776927

The Rabbit Polyclonal anti-ASL antibody has been validated for WB. It is suitable to detect ASL in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Dog, Goat and Saccharomyces cerevisiae. There is 1 publication available.

Quick Overview for ASL antibody (N-Term) (ABIN2776927)

Target: ASL (Argininosuccinate Lyase (ASL))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Dog, Goat, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ASL Antibody

Binding Specificity: N-Term

Sequence: GATAGKLHTG RSRNDQVVTD LRLWMRQTCS TLSGLLWELI RTMVDRAEAE

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ASL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ASL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 464 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ASL antibody (ABIN2776927)

Trevisson, Salviati, Baldoin, Toldo, Casarin, Sacconi, Cesaro, Basso, Burlina: "Argininosuccinate lyase deficiency: mutational spectrum in Italian patients and identification of a novel ASL pseudogene." in: Human mutation, Vol. 28, Issue 7, pp. 694-702, (2007) (PubMed).

Target Details for ASL

Target: ASL (Argininosuccinate Lyase (ASL))

Alternative Name: ASL

Background: ASL is a member of the lyase 1 family. The protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in its gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency.This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.
Alias Symbols: ASAL
Protein Interaction Partner: WDYHV1, ASL, GINS4, NCDN, TUFM, PDHA1, NEDD8, HK1, GDI1, BAG3, OVGP1, HMOX1, FBP1, CSNK2A2, UBC, QARS,
Protein Size: 464

Molecular Weight: 52 kDa

Gene ID: 435

NCBI Accession: NM_000048, NP_000039

UniProt: P04424

Pathways: Response to Growth Hormone Stimulus