POU1F1 antibody (N-Term)

Catalog No. ABIN2777284

This anti-POU1F1 antibody is a Rabbit Polyclonal antibody detecting POU1F1 in WB. Suitable for Human, Mouse, Rat, Dog, Cow, Goat, Horse, Rabbit, Sheep, Guinea Pig and Pig. This Primary Antibody has been cited in 1 publication.

Quick Overview for POU1F1 antibody (N-Term) (ABIN2777284)

Target: POU1F1 (POU Class 1 Homeobox 1 (POU1F1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Goat, Horse, Rabbit, Sheep, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU1F1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POU1F1 Antibody

Binding Specificity: N-Term

Sequence: TPCLYKFPDH TLSHGFPPIH QPLLAEDPTA ADFKQELRRK SKLVEEPIDM

Predicted Reactivity: Cow: 100%, Dog: 92%, Goat: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 100%, Rat: 86%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against POU1F1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POU1F1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 291 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-POU1F1 antibody (ABIN2777284)

Dattani: "Borjeson-Forssman-Lehmann syndrome: a novel pituitary phenotype due to mutation in a novel gene." in: Journal of pediatric endocrinology & metabolism : JPEM, Vol. 16, Issue 9, pp. 1207-9, (2004) (PubMed).

Target Details for POU1F1

Target: POU1F1 (POU Class 1 Homeobox 1 (POU1F1))

Alternative Name: POU1F1

Background: PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP.
Alias Symbols: PIT1, CPHD1, GHF-1, Pit-1, POU1F1a
Protein Interaction Partner: CEBPD, NCOR1, CREBBP, MED1, GATA2, NR1I3, NR1I2, PITX1, JUN, ETS1, NR3C1, VDR, PPARG, PPARA,
Protein Size: 291

Molecular Weight: 33 kDa

Gene ID: 5449

NCBI Accession: NM_000306, NP_000297

UniProt: P28069

Pathways: Regulation of Carbohydrate Metabolic Process