YAF2 antibody (Middle Region)

Catalog No. ABIN309607

This Rabbit Polyclonal antibody specifically detects YAF2 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Rabbit.

Quick Overview for YAF2 antibody (Middle Region) (ABIN309607)

Target: YAF2 (YY1 Associated Factor 2 (YAF2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-YAF2 Antibody

Binding Specificity: Middle Region

Sequence: KGTSTRKPRP VSQLVAQQVT QQFVPPTQSK KEKKDKVEKE KSEKETTSKK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against YAF2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human YAF2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 180 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for YAF2

Target: YAF2 (YY1 Associated Factor 2 (YAF2))

Alternative Name: YAF2

Background: YAF2 interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. YAF2 contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1.The protein encoded by this gene interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. This gene product itself contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two have been confirmed to date.
Alias Symbols: MGC41856
Protein Interaction Partner: SETBP1, XAGE1E, RING1, RNF2, BMI1, GABPB1, CSNK2A2, UBC, CAND1, PCGF1, KDM2B, PCGF5, PCGF6, L3MBTL2, FBRS, BCORL1, BCOR, MGA, CBX3, WDR5, PCGF3, YAF2, USP7, PCGF2, TFDP1, SKP1, HDAC2, E2F6, CSNK2A1, ZFP42, YY1, MYCN,
Protein Size: 180

Molecular Weight: 20 kDa

Gene ID: 10138

NCBI Accession: NM_005748, NP_005739

UniProt: Q8IY57