DDIT3 antibody (N-Term)

Catalog No. ABIN2777369

This Rabbit Polyclonal antibody specifically detects DDIT3 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Sheep, Rabbit, Dog, Guinea Pig, Horse and Goat.

Quick Overview for DDIT3 antibody (N-Term) (ABIN2777369)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Rabbit, Dog, Guinea Pig, Horse, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DDIT3 Antibody

Binding Specificity: N-Term

Sequence: SLPFSFGTLS SWELEAWYED LQEVLSSDEN GGTYVSPPGN EEEESKIFTT

Predicted Reactivity: Cow: 86%, Dog: 86%, Goat: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 93%, Rat: 92%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against DDIT3. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human DDIT3

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Alternative Name: DDIT3

Background: This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Protein Interaction Partner: CCDC153, ATPAF2, ZC3H14, TRIB3, C11orf30, BATF3, VPS37C, AMOTL2, HSD17B14, SNAPC5, HDAC5, HDAC6, TNFSF12, ZBTB25, UBC, LMO2, HDAC1, FOSL2, EP300, CEBPG, C14orf80, GIPC1, BATF, ATF4, PPIB, MAFF, CREB3, FOSL1, NFIL3, MAFG, JUNB, JUN, FOS, EPAS1, DDIT3, DBP,
Protein Size: 192

Molecular Weight: 21 kDa

Gene ID: 1649

NCBI Accession: NP_001181985

UniProt: F8VS99

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis