FOXP3 antibody (N-Term)

Catalog No. ABIN2777561

Cited in 2+ publications. The Rabbit Polyclonal anti-FOXP3 antibody (ABIN2777561) specifically detects FOXP3 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Pig, Rabbit, Horse, Dog, Sheep and Guinea Pig samples.

Quick Overview for FOXP3 antibody (N-Term) (ABIN2777561)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Horse, Dog, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FOXP3 Antibody

Binding Specificity: N-Term

Sequence: PHFMHQLSTV DAHARTPVLQ VHPLESPAMI SLTPPTTATG VFSLKARPGL

Predicted Reactivity: Cow: 100%, Dog: 79%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 86%, Rat: 86%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against FOXP3. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FOXP3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 431 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FOXP3 antibody (ABIN2777561)

Kawabata, Ohta, Seiler, Pyle, Ishiguro, Zhang, Becker, Troyer, Tamura: "Naïve rat umbilical cord matrix stem cells significantly attenuate mammary tumor growth through modulation of endogenous immune responses." in: Cytotherapy, Vol. 15, Issue 5, pp. 586-97, (2013) (PubMed).

Takahata, Nomura, Takada, Ohga, Furuno, Hikino, Nakayama, Sakaguchi, Hara: "CD25+CD4+ T cells in human cord blood: an immunoregulatory subset with naive phenotype and specific expression of forkhead box p3 (Foxp3) gene." in: Experimental hematology, Vol. 32, Issue 7, pp. 622-9, (2004) (PubMed).

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: FOXP3

Background: Forkhead box protein P3 (FOXP3, Scurfin, Zinc finger protein JM2) encodes a novel member of the forkhead family of transcription factors. It presumably represses transcription, playing a paramount role in determining the amplitude of the response of CD4 T cells to activation
Alias Symbols: JM2, AIID, IPEX, PIDX, XPID, DIETER
Protein Interaction Partner: PRR20A, RBPMS, STUB1, UBC, HSPA1A, IKZF3, GATA3, RUNX1, RELA, NFATC1, HIST1H1B, HDAC1, KAT8, REL, IKZF4, NFAT5, NFATC2, HDAC4, HDAC2, HDAC7, KAT5, HDAC9,
Protein Size: 431

Molecular Weight: 47 kDa

Gene ID: 50943

NCBI Accession: NM_014009, NP_054728

UniProt: Q9BZS1

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation