PHAP1 antibody (Middle Region)

Catalog No. ABIN2778667

This anti-PHAP1 antibody is a Rabbit Polyclonal antibody detecting PHAP1 in WB and IHC. Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Rabbit and Sheep.

Quick Overview for PHAP1 antibody (Middle Region) (ABIN2778667)

Target: PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PHAP1 Antibody

Binding Specificity: Middle Region

Sequence: FNCEVTNLND YRENVFKLLP QLTYLDGYDR DDKEAPDSDA EGYVEGLDDE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 100%, Sheep: 86%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against ANP32A. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ANP32A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 249 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PHAP1

Target: PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))

Alternative Name: ANP32A

Background: ANP32A is a novel potent heat-stable inhibitor protein of protein phosphatase 2A. It may play a key role in self-renewing cell populations where it may act in the nucleus to limit their sensitivity to transformation. Being a tumor suppressor, ANP32A represses cell growth through inhibition of transcription by blocking acetylation and phosphorylation of histone H3 and initiating its proapoptotic activity.
Alias Symbols: LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1
Protein Interaction Partner: UBC, rev, PDLIM5, PLIN3, ARPC1B, OXSR1, EIF4A3, VBP1, TPD52L2, SSB, SRP14, RPA2, PKM, BAG1, SOX2, PCBP1, ESR1, THRB, PPARG, PGR, KPNA6, KPNA5, KPNA1, SUMO2, CHAF1A, HIST1H4A, HIST1H3A, HIST2H2BE, HIST2H2AC, SET, MEPCE, ELAVL1, ADAP1, NSFL1C, HSPB3, AXIN1,
Protein Size: 249

Molecular Weight: 29 kDa

Gene ID: 8125

NCBI Accession: NM_006305, NP_006296

UniProt: P39687