Heterogeneous Nuclear Ribonucleoprotein D (AU-Rich Element RNA Binding Protein 1, 37kDa) (HNRNPD) (N-Term) antibody

Details for Product No. ABIN2778694
Antigen
Epitope
N-Term
24
17
11
6
4
4
4
3
3
3
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
135
54
39
7
4
3
2
2
2
2
1
1
1
Host
Rabbit
132
3
Clonality
Polyclonal
Conjugate
Un-conjugated
10
6
6
4
4
4
4
4
4
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
109
65
62
42
15
11
9
4
3
2
Options
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HNRPD
Sequence AESEGAKIDA SKNEEDEGHS NSSPRHSEAA TAQREEWKMF IGGLSWDTTK
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 77%, Horse: 93%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against HNRPD. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Antigen
Alternative Name HNRPD (HNRNPD Antibody Abstract)
Background HNRPD belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
Alias Symbols: P37, AUF1, AUF1A, HNRPD, hnRNPD0
Protein Interaction Partner: NCL, FUS, KAT2B, SUMO2, SUMO3, IVNS1ABP, STAU1, UBC, NEDD8, MDM2, ERG, RPA3, RPA2, RPA1, ASB2, EED, rev, ERI1, RPS12, PTBP1, PAFAH1B3, WNK1, IPO11, EIF3K, KHSRP, DENR, PARK2, DGCR2, GTF3C3, PDLIM7, UQCRC1, HIRA, SNAPC4, SLC3A2, SDF2, COG7, TMEM261, ABCC10
Protein Size: 355
Molecular Weight 39 kDa
Gene ID 3184
NCBI Accession NM_031370, NP_112738
UniProt Q14103
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 355 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Heterogeneous Nuclear Ribonucleoprotein D (AU-Rich Element RNA Binding Protein 1, 37kDa) (HNRNPD) (N-Term) antibody (ABIN2778694) WB Suggested Anti-HNRPD Antibody Titration: 1.25ug/ml ELISA Titer: 1:62500 Positi...
Image no. 2 for anti-Heterogeneous Nuclear Ribonucleoprotein D (AU-Rich Element RNA Binding Protein 1, 37kDa) (HNRNPD) (N-Term) antibody (ABIN2778694) Rabbit Anti-HNRPD Antibody Paraffin Embedded Tissue: Human Intestine Cellular Data: ...
Image no. 3 for anti-Heterogeneous Nuclear Ribonucleoprotein D (AU-Rich Element RNA Binding Protein 1, 37kDa) (HNRNPD) (N-Term) antibody (ABIN2778694) WB Suggested Anti-HNRPD Antibody Titration: 1.25 µg/mL ELISA Titer: 1:62500 Positiv...
Product cited in: Dhakras, Hajarnis, Taylor, Curthoys: "cAMP-dependent stabilization of phosphoenolpyruvate carboxykinase mRNA in LLC-PK1-F+ kidney cells." in: American journal of physiology. Renal physiology, Vol. 290, Issue 2, pp. F313-8, 2006 (PubMed).