Splicing factor U2AF 26 kDa subunit (U2AF1L4) (N-Term) antibody

Catalog No. ABIN2778747

The Rabbit Polyclonal anti- antibody has been validated for WB. It is suitable to detect in samples from Human, Dog, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Goat.

Quick Overview for Splicing factor U2AF 26 kDa subunit (U2AF1L4) (N-Term) antibody (ABIN2778747)

Target: Splicing factor U2AF 26 kDa subunit (U2AF1L4)

Reactivity: Human, Dog, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Sequence: KIGVCRHGDR CSRLHNKPTF SQTIVLLNLY RNPQNTAQTA DGSHCHVSDV

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 79%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against U2AF1L4. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human U2AF1L4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: Splicing factor U2AF 26 kDa subunit (U2AF1L4)

Alternative Name: U2AF1L4

Background: U2AF1L4 is a RNA-binding protein that function as a pre-mRNA splicing factor. It plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. It acts by enhancing the binding of U2AF2 to weak pyrimidine tracts and also participates in the regulation of alternative pre-mRNA splicing. It activates exon 5 skipping of PTPRC during T-cell activation, an event reversed by GFI1. U2AF1L4 binds to RNA at the AG dinucleotide at the 3'-splice site.
Protein Interaction Partner: MDM2, SUZ12, UBC, ELAVL1, GIGYF2,
Protein Size: 220

Molecular Weight: 24 kDa

Gene ID: 199746

UniProt: Q8WU68