Splicing factor U2AF 26 kDa subunit (U2AF1L4) (N-Term) antibody

Catalog No. ABIN2778747

The Rabbit Polyclonal anti-not_set antibody has been validated for WB. It is suitable to detect not_set in samples from Human, Dog, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Goat.

Quick Overview for Splicing factor U2AF 26 kDa subunit (U2AF1L4) (N-Term) antibody (ABIN2778747)

Target: Splicing factor U2AF 26 kDa subunit (U2AF1L4)

Reactivity: Human, Dog, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Sequence: KIGVCRHGDR CSRLHNKPTF SQTIVLLNLY RNPQNTAQTA DGSHCHVSDV

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 79%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against U2AF1L4. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human U2AF1L4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: Splicing factor U2AF 26 kDa subunit (U2AF1L4)

Alternative Name: U2AF1L4

Background: U2AF1L4 is a RNA-binding protein that function as a pre-mRNA splicing factor. It plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. It acts by enhancing the binding of U2AF2 to weak pyrimidine tracts and also participates in the regulation of alternative pre-mRNA splicing. It activates exon 5 skipping of PTPRC during T-cell activation, an event reversed by GFI1. U2AF1L4 binds to RNA at the AG dinucleotide at the 3'-splice site.
Protein Interaction Partner: MDM2, SUZ12, UBC, ELAVL1, GIGYF2,
Protein Size: 220

Molecular Weight: 24 kDa

Gene ID: 199746

UniProt: Q8WU68