EIF4E antibody (C-Term)

Catalog No. ABIN2778784

This Rabbit Polyclonal antibody specifically detects EIF4E in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Rabbit, Dog, Guinea Pig and Horse.

Quick Overview for EIF4E antibody (C-Term) (ABIN2778784)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Rabbit, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EIF4E Antibody

Binding Specificity: C-Term

Sequence: TECENREAVT HIGRVYKERL GLPPKIVIGY QSHADTATKS GSTTKNRFVV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF4E. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EIF4E

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 217 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: EIF4E

Background: All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CBP, EIF4E1, EIF4EL1, EIF4F, MGC111573
Protein Interaction Partner: EIF4EBP1, UBXN11, EIF4ENIF1, KANK2, TRIM22, EIF4EBP3, TDO2, TRIM27, EIF4G1, EIF4EBP2, EIF3B, RPS6KB1, MTOR, PML, NANS, PROSC, AHSA1, PEPD, EIF6, HSPD1, HSPA9, TYMP, CAPN1, ASS1, GEMIN5, PRDM4, CD83, UBC, ITGA4, APP, EIF4H, RPTOR, EIF4A1, RPS6KA5, HSPB2, E
Protein Size: 217

Molecular Weight: 25 kDa

Gene ID: 1977

NCBI Accession: NM_001968, NP_001959

UniProt: P06730

Pathways: BCR Signaling