ILK antibody (C-Term)

Catalog No. ABIN2779334

The Rabbit Polyclonal anti-ILK antibody has been validated for WB. It is suitable to detect ILK in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Goat, Guinea Pig, Horse, Rabbit and Sheep.

Quick Overview for ILK antibody (C-Term) (ABIN2779334)

Target: ILK (Integrin-Linked Kinase (ILK))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Goat, Guinea Pig, Horse, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ILK Antibody

Binding Specificity: C-Term

Sequence: MKVALEGLRP TIPPGISPHV CKLMKICMNE DPAKRPKFDM IVPILEKMQD

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 82%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ILK. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human ILK

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ILK

Target: ILK (Integrin-Linked Kinase (ILK))

Alternative Name: ILK

Background: Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Alias Symbols: ILK-2, P59
Protein Interaction Partner: UBC, TRIM27, PARVG, PARVA, RICTOR, ITGB1, MTOR, AKT1, FKBP5, HSPH1, FAF1, CARM1, UGP2, PPAT, FBXO6, ACACA, LIMS2, RHOT1, FANCI, ATAD3A, HEATR1, CLN6, TRAFD1, ZNF568, TIMM50, RHOT2, TRABD, LPCAT1, VKORC1, RPL13, ABCE1, RBBP7, RAF1, SDF4, SEC61A1, PARVB, AK
Protein Size: 452

Molecular Weight: 49 kDa

Gene ID: 3611

NCBI Accession: NM_004517, NP_004508

UniProt: Q13418

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration