IRF6 antibody (Middle Region)

Catalog No. ABIN2779406

This Rabbit Polyclonal antibody specifically detects IRF6 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Sheep, Zebrafish (Danio rerio) and Pigand has been mentioned in 1 publication.

Quick Overview for IRF6 antibody (Middle Region) (ABIN2779406)

Target: IRF6 (Interferon Regulatory Factor 6 (IRF6))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Sheep, Zebrafish (Danio rerio), Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IRF6 Antibody

Binding Specificity: Middle Region

Sequence: IPVVARMIYE MFSGDFTRSF DSGSVRLQIS TPDIKDNIVA QLKQLYRILQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against IRF6. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human IRF6

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 467 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-IRF6 antibody (ABIN2779406)

Iwata, Suzuki, Pelikan, Ho, Sanchez-Lara, Urata, Dixon, Chai: "Smad4-Irf6 genetic interaction and TGF?-mediated IRF6 signaling cascade are crucial for palatal fusion in mice." in: Development (Cambridge, England), Vol. 140, Issue 6, pp. 1220-30, (2013) (PubMed).

Target Details for IRF6

Target: IRF6 (Interferon Regulatory Factor 6 (IRF6))

Alternative Name: IRF6

Background: IRF6 is a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in its gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation.The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: LPS, OFC6, PIT, PPS, VWS, VWS1
Protein Interaction Partner: HHV8GK18_gp81, UBC, BNC2, IRF5, IRF8, ZBTB3, RFX3, TLX2, LBP,
Protein Size: 467

Molecular Weight: 53 kDa

Gene ID: 3664

NCBI Accession: NM_006147, NP_006138

UniProt: O14896