CIITA antibody (Middle Region)

Catalog No. ABIN2780332

This Rabbit Polyclonal antibody specifically detects CIITA in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Pig and Rabbit.

Quick Overview for CIITA antibody (Middle Region) (ABIN2780332)

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CIITA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CIITA Antibody

Binding Specificity: Middle Region

Sequence: YNKFTAAGAQ QLAASLRRCP HVETLAMWTP TIPFSVQEHL QQQDSRISLR

Predicted Reactivity: Cow: 79%, Dog: 86%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against CIITA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CIITA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1130 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CIITA

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Alternative Name: CIITA

Background: CIITA is a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the 'master control factor' for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction.This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the 'master control factor' for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: C2TA, CIITA IV, MHC2TA, NLRA, CIITAIV
Protein Interaction Partner: PSMC5, CUL3, TAF9, GTF2B, PSMC3, PSMC2, SIN3B, HDAC2, GSK3B, HDAC5, HDAC4, SMARCA4, RFX5, HDAC1, ZXDC, TAF6, KAT2B, RFXANK, NCOA1, XPO1, PRKACA, NFYB, MAPK3, MAPK1, CDK1, HIST4H4, POLR2A, ACTB,
Protein Size: 1130

Molecular Weight: 123 kDa

Gene ID: 4261

NCBI Accession: NM_000246, NP_000237

UniProt: P33076

Pathways: Cancer Immune Checkpoints