RFX5 antibody (N-Term)

Catalog No. ABIN2780342

This Rabbit Polyclonal antibody specifically detects RFX5 in WB. It exhibits reactivity toward Human, Rat, Dog, Guinea Pig, Horse, Rabbit and Pig.

Quick Overview for RFX5 antibody (N-Term) (ABIN2780342)

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Reactivity: Human, Rat, Dog, Guinea Pig, Horse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFX5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RFX5 Antibody

Binding Specificity: N-Term

Sequence: MAEDEPDAKS PKTGGRAPPG GAEAGEPTTL LQRLRGTISK AVQNKVEGIL

Predicted Reactivity: Dog: 86%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Pig: 86%, Rabbit: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against RFX5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RFX5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 616 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RFX5

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Alternative Name: RFX5

Background: RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. RFX is a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS, MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS, MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.
Protein Interaction Partner: UBC, MRPL53, SLIRP, SRPRB, CDV3, LSM7, RPS19, RBMS1, NDUFA7, NDUFA2, ANKRA2, RFXANK, MLLT1, HDAC4, CIITA, HDAC2, RFXAP,
Protein Size: 616

Molecular Weight: 68 kDa

Gene ID: 5993

NCBI Accession: NM_001025603, NP_001020774

UniProt: P48382