TCF3 antibody (N-Term)

Catalog No. ABIN2780485

Product Details anti-TCF3 Antibody

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MNQPQRMAPV GTDKELSDLL DFSMMFPLPV TNGKGRPASL AGAQFGGSGL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 77%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TCF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TCF3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 654 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TCF3

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Alternative Name: TCF3 (TCF3 Products)

Background: TCF3 contains 1 basic helix-loop-helix (bHLH) domain. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. TCF3 binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
Alias Symbols: E2A, E47, ITF1, VDIR, TCF-3, bHLHb21
Protein Interaction Partner: ID3, FAM115A, TLE1, RNF14, UBC, SCX, MAPKAPK2, MAPKAPK3, TCF21, Ube2i, ID2, FUS, CTNNB1, RPL37, MAX, USF1, TCF12, TCF3, MYOD1, Rufy1, Tfap4, Rpa1, Tbx6, Ncl, Twist2, SUPT3H, TRRAP, KAT2A, TADA2A, FOXH1, ELAVL1, TCF24, SETSIP, FLG2, BHLHA15, MYL6B, TIMM50,
Protein Size: 654

Molecular Weight: 68 kDa

Gene ID: 6929

NCBI Accession: NM_003200, NP_003191

UniProt: P15923

Pathways: WNT Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response