TCF3 antibody (pThr355)

Catalog No. ABIN7138838

This Rabbit Polyclonal antibody specifically detects TCF3 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for TCF3 antibody (pThr355) (ABIN7138838)

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-TCF3 Antibody

Binding Specificity: pThr355

Purpose: Phospho-TCF3 (Thr355) Antibody

Cross-Reactivity: Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of threonine 355 (P-S-T(p)-P-V) derived from Human E2A .

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for TCF3

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Alternative Name: TCF3

Background:

Background:

E2A is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.

Kamps M.P., Cell 60:547-555(1990).
Nourse J., Cell 60:535-545(1990).
Grimwood J., Nature 428:529-535(2004).

Aliases: AGM8 antibody, bHLHb21 antibody, Class B basic helix-loop-helix protein 21 antibody, E12 antibody, E2A antibody, E2A immunoglobulin enhancer binding factors E12/E47 antibody, E47 antibody, Helix loop helix protein HE47 antibody, Immunoglobulin enhancer-binding factor E12/E47 antibody, Immunoglobulin transcription factor 1 antibody, ITF1 antibody, Kappa-E2-binding factor antibody, MGC129647 antibody, MGC129648 antibody, Negative vitamin D response element binding protein antibody, NOL1-TCF3 fusion antibody, TCF-3 antibody, Tcf3 antibody, TFE2_HUMAN antibody, transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) antibody, Transcription factor 3 antibody, transcription factor 3 variant 3 antibody, Transcription factor E2-alpha antibody, Transcription factor ITF-1 antibody, VDIR antibody, VDR interacting repressor antibody, vitamin D receptor-interacting repressor antibody

UniProt: P15923

Pathways: WNT Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response