NR2F2 antibody (N-Term)

Catalog No. ABIN2781130

The Rabbit Polyclonal anti-NR2F2 antibody has been validated for WB and IHC. It is suitable to detect NR2F2 in samples from Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for NR2F2 antibody (N-Term) (ABIN2781130)

Target: NR2F2 (Nuclear Receptor Subfamily 2, Group F, Member 2 (NR2F2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR2F2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NR2F2 Antibody

Binding Specificity: N-Term

Sequence: QDEVPGSQGS QASQAPPVPG PPPGAPHTPQ TPGQGGPAST PAQTAAGGQG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against NR2F2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NR2F2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 414 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NR2F2

Target: NR2F2 (Nuclear Receptor Subfamily 2, Group F, Member 2 (NR2F2))

Alternative Name: NR2F2

Background: NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing transcription. However, in certain contexts, NR2F2 stimulates transcription directly. NR2F2, MyoD and p300 interact in a competitive manner, and that increasing amounts of NR2F2 have the ability to reduce the interaction between myoD and p300 invitro. NR2F2 post-transcriptionally regulates myoD activity/function, and that crosstalk between orphan nuclear receptors and the myogenic bHLH proteins has functional consequences for differentiation.
Alias Symbols: ARP1, COUP-TFII, COUPTFB, MGC117452, SVP40, TFCOUP2, NF-E3, NR2F1, COUPTFII
Protein Interaction Partner: NSD1, BCL11A, NR2F2, BIK, SETD7, FAM46A, HIPK3, UBC, TFAP4, Cebpb, SMARCAD1, NR2F6, POU5F1, NR3C1, PHB2, TRIP4, PIAS1, BCL11B, TRIM24, EP300, SQSTM1, HDAC1, NCOR2, MYOD1, LCK,
Protein Size: 414

Molecular Weight: 45 kDa

Gene ID: 7026

NCBI Accession: NM_021005, NP_066285

UniProt: P24468

Pathways: Steroid Hormone Mediated Signaling Pathway