RRM1 antibody (C-Term)

Catalog No. ABIN2782438

The Rabbit Polyclonal anti-RRM1 antibody has been validated for WB. It is suitable to detect RRM1 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Rabbit, Horse, Guinea Pig and Cow. There are 3+ publications available.

Quick Overview for RRM1 antibody (C-Term) (ABIN2782438)

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Rabbit, Horse, Guinea Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RRM1 Antibody

Binding Specificity: C-Term

Sequence: MHFYGWKQGL KTGMYYLRTR PAANPIQFTL NKEKLKDKEK VSKEEEEKER

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against RRM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RRM1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 792 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RRM1 antibody (ABIN2782438)

Zhu, Wonganan, Lansakara-P, OMary, Li, Cui: "The effect of the acid-sensitivity of 4-(N)-stearoyl gemcitabine-loaded micelles on drug resistance caused by RRM1 overexpression." in: Biomaterials, Vol. 34, Issue 9, pp. 2327-39, (2013) (PubMed).

Chung, Sandoval, Sloat, Lansakara-P, Cui: "Stearoyl gemcitabine nanoparticles overcome resistance related to the over-expression of ribonucleotide reductase subunit M1." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 157, Issue 1, pp. 132-40, (2012) (PubMed).

Wonganan, Chung, Zhu, Kiguchi, Digiovanni, Cui: "Silencing of ribonucleotide reductase subunit M1 potentiates the antitumor activity of gemcitabine in resistant cancer cells." in: Cancer biology & therapy, Vol. 13, Issue 10, pp. 908-14, (2012) (PubMed).

Target Details for RRM1

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Alternative Name: RRM1

Background: RRM1 is one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. Its gene is located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. Its gene may play a role in malignancies and disease that involve this region.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1), with the 3' end of STIM1 situated 1.6 kb from the 5' end of this gene.
Alias Symbols: R1, RIR1, RR1
Protein Interaction Partner: UBC, RRM1, SUMO2, RNF2, BMI1, PPIB, TNPO1, FBXO6, PPP1CC, TMEM177, DNAJB11, SUCLA2, SGSH, NDUFV1, EEF2, DNASE2, COPS5, CDK2, RRM2, RRM2B, KAT5, LRIF1, YWHAG,
Protein Size: 792

Molecular Weight: 90 kDa

Gene ID: 6240

NCBI Accession: NM_001033, NP_001024

UniProt: P23921

Pathways: Positive Regulation of Endopeptidase Activity