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MELK antibody (Middle Region)

This Rabbit Polyclonal antibody specifically detects MELK in WB. It exhibits reactivity toward Human, Rat, Cow, Horse, Guinea Pig, Dog and Rabbit.
Catalog No. ABIN2782538

Quick Overview for MELK antibody (Middle Region) (ABIN2782538)

Target

See all MELK Antibodies
MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reactivity

  • 64
  • 21
  • 16
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
Human, Rat, Cow, Horse, Guinea Pig, Dog, Rabbit

Host

  • 66
  • 7
Rabbit

Clonality

  • 64
  • 9
Polyclonal

Conjugate

  • 42
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MELK antibody is un-conjugated

Application

  • 45
  • 29
  • 14
  • 13
  • 13
  • 10
  • 9
  • 7
  • 7
  • 5
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 15
    • 8
    • 8
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region

    Sequence

    AVKNEEYFMF PEPKTPVNKN QHKREILTTP NRYTTPSKAR NQCLKETPIK

    Predicted Reactivity

    Cow: 79%, Dog: 93%, Guinea Pig: 80%, Horse: 100%, Human: 100%, Rabbit: 87%, Rat: 93%

    Characteristics

    This is a rabbit polyclonal antibody against MELK. It was validated on Western Blot using a cell lysate as a positive control.

    Purification

    Affinity Purified

    Immunogen

    The immunogen is a synthetic peptide directed towards the middle region of human MELK
  • Application Notes

    Optimal working dilutions should be determined experimentally by the investigator.

    Comment

    Antigen size: 651 AA

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze-thaw cycles.

    Storage

    -20 °C

    Storage Comment

    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target

    MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

    Alternative Name

    MELK

    Background

    The protein MELK phosphorylates ZNF622 and may contribute to its redirection to the nucleus. Also it may be involved in the inhibition of spliceosome assembly during mitosis.
    Alias Symbols: HPK38, KIAA0175
    Protein Interaction Partner: UBC, YGR054W, Acaca, CSN1S1, MELK, MBP, LMNB1, BABAM1, ZNF622, SF3B1, EIF2S1, CDC5L, PPP1R8, HIST1H1B, CDC25B,
    Protein Size: 651

    Molecular Weight

    75 kDa

    Gene ID

    9833

    NCBI Accession

    NM_014791, NP_055606

    UniProt

    Q14680
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