HRK antibody (N-Term)

Catalog No. ABIN2782598

The Rabbit Polyclonal anti-HRK antibody has been validated for WB. It is suitable to detect HRK in samples from Human, Mouse, Rat, Cow, Dog and Rabbit. There is 1 publication available.

Quick Overview for HRK antibody (N-Term) (ABIN2782598)

Target: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HRK Antibody

Binding Specificity: N-Term

Sequence: MCPCPLHRGR GPPAVCACSA GRLGLRSSAA QLTAARLKAL GDELHQRTMW

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 83%, Rabbit: 100%, Rat: 83%

Characteristics: This is a rabbit polyclonal antibody against HRK. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HRK

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 91 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HRK antibody (ABIN2782598)

Lucas, Milani, Butterworth, Carmell, Scott, Clark, Cohen, Varadarajan: "High CIP2A levels correlate with an antiapoptotic phenotype that can be overcome by targeting BCL-XL in chronic myeloid leukemia." in: Leukemia, Vol. 30, Issue 6, pp. 1273-81, (2016) (PubMed).

Target Details for HRK

Target: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Alternative Name: HRK

Background: Activator of apoptosis Hrk regulates apoptosis through interaction with death-repressor proteins Bcl-2 and Bcl-X(L). The HRK protein lacks significant homology to other BCL2 family members except for an 8-amino acid region that was similar to the BCL2 hom
Alias Symbols: DP5, HARAKIRI
Protein Interaction Partner: BCL2L1, ELAVL1, BAD, BCL2L2, C1QBP, BCL2A1, BCL2, MCL1, BNC1,
Protein Size: 91

Molecular Weight: 10 kDa

Gene ID: 8739

NCBI Accession: NM_003806, NP_003797

UniProt: O00198