COX15 antibody (N-Term)

Catalog No. ABIN2782632

This anti-COX15 antibody is a Rabbit Polyclonal antibody detecting COX15 in IHC and WB. Suitable for Human, Rat, Mouse, Dog, Rabbit, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse and Saccharomyces cerevisiae. This Primary Antibody has been cited in 1 publication.

Quick Overview for COX15 antibody (N-Term) (ABIN2782632)

Target: COX15 (Cytochrome C Oxidase Assembly Homolog 15 (COX15))

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX15 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-COX15 Antibody

Binding Specificity: N-Term

Sequence: DWHLIKEMKP PTSQEEWEAE FQRYQQFPEF KILNHDMTLT EFKFIWYMEY

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 86%, Rat: 93%, Yeast: 82%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against COX15. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human COX15

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 388 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-COX15 antibody (ABIN2782632)

Swenson, Cannon, Harris, Taylor, Fox, Khalimonchuk: "Analysis of Oligomerization Properties of Heme a Synthase Provides Insights into Its Function in Eukaryotes." in: The Journal of biological chemistry, Vol. 291, Issue 19, pp. 10411-25, (2016) (PubMed).

Target Details for COX15

Target: COX15 (Cytochrome C Oxidase Assembly Homolog 15 (COX15))

Alternative Name: COX15

Background: Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane.Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane. Alternative splicing of this gene generates several transcript variants diverging in the 3' region including alternate poly A sites. In total, 2 different isoforms are encoded by these variants.
Alias Symbols: -
Protein Interaction Partner: UBC, CLN3, MRPS14, NDUFA12, TOMM7, MRPL15, LAMTOR3, SURF1, NDUFS8, NDUFS6, NDUFS4, NDUFA9, CYC1,
Protein Size: 388

Molecular Weight: 44 kDa

Gene ID: 1355

NCBI Accession: NM_004376, NP_004367