HYOU1 antibody (Middle Region)

Catalog No. ABIN2782712

Product Details anti-HYOU1 Antibody

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HYOU1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DREVQYLLNK AKFTKPRPRP KDKNGTRAEP PLNASASDQG EKVIPPAGQT

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HYOU1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HYOU1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 999 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HYOU1 antibody (ABIN2782712)

Du, Rong, Hui, Peng, Jin, Li, Wang, Li: "Expression and function of HSP110 family in mouse testis after vasectomy." in: Asian journal of andrology, Vol. 19, Issue 3, pp. 355-361, (2016) (PubMed).

Target Details for HYOU1

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Alternative Name: HYOU1 (HYOU1 Products)

Background: The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.
Alias Symbols: DKFZp686N08236, ORP150, Grp170, HSP12A
Protein Interaction Partner: CLU, HSPA8, UBC, MDM2, RNF2, EZH2, KIF13A, ARMCX3, GANAB, BCAR1, ATP6V1F, XRCC5, MCM7, MCM2, MSH6, XRCC6, ATP6V1B2, BIN1, UBD, env, OS9, NUP37, POLR1C, FBXO6, CDK2, SIRT7, SARM1, H2AFX, IKBKE, EXT2,
Protein Size: 999

Molecular Weight: 108 kDa

Gene ID: 10525

NCBI Accession: NM_006389, NP_006380

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome