HYOU1 antibody

Catalog No. ABIN2868628

Product Details anti-HYOU1 Antibody

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HYOU1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~170 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Recombinant Full length GRP170 Protein

Clone: 6E3-2C3

Isotype: IgG2b

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2868628 was sufficient for detection of GRP170 in 20 μg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for HYOU1

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Alternative Name: GRP170 (HYOU1 Products)

Synonyms: GRP-170 antibody, Grp170 antibody, HSP12A antibody, ORP-150 antibody, ORP150 antibody, AI415631 antibody, CBP-140 antibody, Cab140 antibody, Orp150 antibody, id:ibd5056 antibody, wu:fd14c05 antibody, zgc:56019 antibody, HYOU1 antibody, orp150 antibody, GRP170 antibody, hypoxia up-regulated 1 antibody, hypoxia up-regulated protein 1 antibody, hypoxia up-regulated 1 S homeolog antibody, HYOU1 antibody, Hyou1 antibody, hyou1 antibody, LOC100380749 antibody, hyou1.S antibody

Background: GRP170, also known as ORP150, is the largest member of glucose-regulated Antibodys, and acts as a human chaperone Antibody. It is thought to play an important role in Antibody folding and secretion in the ER. Suppression of the Antibody is associated with accelerated apoptosis, therefore having an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumor immune response, which will hopefully lead to therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumor immune responses (2).

Gene ID: 10525

NCBI Accession: NP_006389

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome