ALG2 antibody (C-Term)

Catalog No. ABIN2783587

This anti-ALG2 antibody is a Rabbit Polyclonal antibody detecting ALG2 in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae, Goat and Zebrafish (Danio rerio). This Primary Antibody has been cited in 2+ publications.

Quick Overview for ALG2 antibody (C-Term) (ABIN2783587)

Target: ALG2 (Asparagine-Linked Glycosylation 2, alpha-1,3-Mannosyltransferase Homolog (ALG2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae, Goat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALG2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ALG2 Antibody

Binding Specificity: C-Term

Sequence: QSDLGQYVTF LRSFSDKQKI SLLHSCTCVL YTPSNEHFGI VPLEAMYMQC

Predicted Reactivity: Cow: 100%, Dog: 93%, Goat: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ALG2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ALG2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 416 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ALG2 antibody (ABIN2783587)

Cossins, Belaya, Hicks, Salih, Finlayson, Carboni, Liu, Maxwell, Zoltowska, Farsani, Laval, Seidhamed, Donnelly, Bentley, McGowan, Müller, Palace, Lochmüller, Beeson: "Congenital myasthenic syndromes due to mutations in ALG2 and ALG14." in: Brain : a journal of neurology, Vol. 136, Issue Pt 3, pp. 944-56, (2013) (PubMed).

Rind, Schmeiser, Thiel, Absmanner, Lübbehusen, Hocks, Apeshiotis, Wilichowski, Lehle, Körner: "A severe human metabolic disease caused by deficiency of the endoplasmatic mannosyltransferase hALG11 leads to congenital disorder of glycosylation-Ip." in: Human molecular genetics, Vol. 19, Issue 8, pp. 1413-24, (2010) (PubMed).

Target Details for ALG2

Target: ALG2 (Asparagine-Linked Glycosylation 2, alpha-1,3-Mannosyltransferase Homolog (ALG2))

Alternative Name: ALG2

Background: ALG2 is a member of the glycosyltransferase 1 family. It acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii).This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CDGIi, FLJ14511, hALPG2, NET38
Protein Interaction Partner: UBC, PLSCR3, SHISA5, PDCD6IP, ALG2, VPS28, PTPN23, SEC31A, TSG101, LAMP1, HSP90AA1, PTK2B, ANXA11, ANXA7, VPS37A, PEF1, SRI,
Protein Size: 416

Molecular Weight: 47 kDa

Gene ID: 85365

NCBI Accession: NM_033087, NP_149078

UniProt: Q9H553