SRD5A3 antibody (N-Term)

Catalog No. ABIN2783928

This Rabbit Polyclonal antibody specifically detects SRD5A3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog, Horse and Saccharomyces cerevisiaeand has been mentioned in 1 publication.

Quick Overview for SRD5A3 antibody (N-Term) (ABIN2783928)

Target: SRD5A3 (Steroid 5 alpha-Reductase 3 (SRD5A3))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRD5A3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SRD5A3 Antibody

Binding Specificity: N-Term

Sequence: GLLPGCAIFQ DLIRYGKTKC GEPSRPAACR AFDVPKRYFS HFYIISVLWN

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 89%

Characteristics: This is a rabbit polyclonal antibody against SRD5A3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SRD5A3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 318 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SRD5A3 antibody (ABIN2783928)

Uemura, Tamura, Chung, Honma, Okuyama, Nakamura, Nakagawa: "Novel 5 alpha-steroid reductase (SRD5A3, type-3) is overexpressed in hormone-refractory prostate cancer." in: Cancer science, Vol. 99, Issue 1, pp. 81-6, (2007) (PubMed).

Target Details for SRD5A3

Target: SRD5A3 (Steroid 5 alpha-Reductase 3 (SRD5A3))

Alternative Name: SRD5A3

Background: SRD5A3 belongs to the steroid 5-alpha reductase family and converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT).
Alias Symbols: FLJ13352, SRD5A2L, CDG1P, CDG1Q, KRIZI, SRD5A2L1
Protein Interaction Partner: NUDT15, ADK, DFG10,
Protein Size: 318

Molecular Weight: 36 kDa

Gene ID: 79644

NCBI Accession: NM_024592, NP_078868

UniProt: Q9H8P0

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis