Dynamin 1 antibody (Middle Region)

Catalog No. ABIN2784624

This anti-Dynamin 1 antibody is a Rabbit Polyclonal antibody detecting Dynamin 1 in WB and IHC. Suitable for Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for Dynamin 1 antibody (Middle Region) (ABIN2784624)

Target: Dynamin 1 (DNM1)

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dynamin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Dynamin 1 Antibody

Binding Specificity: Middle Region

Sequence: PPVDDSWLQV QSVPAGRRSP TSSPTPQRRA PAVPPARPGS RGPAPGPPPA

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against DNM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DNM1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 851 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: DNM1

Background: DNM1 is a member of the dynamin subfamily of GTP-binding proteins. The protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.
Alias Symbols: DNM
Protein Interaction Partner: BIN1, SLA1, UBC, GRB2, AMPH, NCL, FANCD2, IRF2BPL, TBC1D2, NPM3, SNRPA1, SNX9, Fcho2, IQCB1, NCK2, NCK1, Asap1, SH3BP4, DNM1, PTK2B, PIAS1, SUMO1, UBE2I, SRC, ITSN1, SH3GL2, DYRK1A, CDK5, ARRB1, Ap2a1, SH3KBP1, DNM2, ADAM2, UBASH3A, KIAA1377, GRIN1, MED31
Protein Size: 851

Molecular Weight: 95 kDa

Gene ID: 1759

NCBI Accession: NM_004408, NP_004399

UniProt: P39053

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling