UBE2F antibody (N-Term)

Catalog No. ABIN2784961

This anti-UBE2F antibody is a Rabbit Polyclonal antibody detecting UBE2F in WB and IHC. Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for UBE2F antibody (N-Term) (ABIN2784961)

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-UBE2F Antibody

Binding Specificity: N-Term

Sequence: MLTLASKLKR DDGLKGSRTA ATASDSTRRV SVRDKLLVKE VAELEANLPC

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against UBE2F. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2F

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 185 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: UBE2F

Background: UBE2F belongs to the ubiquitin-conjugating enzyme family. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.
Alias Symbols: MGC18120, NCE2
Protein Interaction Partner: CUL1, UBC, NEDD8, DCUN1D3, DCUN1D2, DCUN1D1, RNF7, CUL5, S100A11, vif, ELAVL1, RNF34,
Protein Size: 185

Molecular Weight: 21 kDa

Gene ID: 140739

NCBI Accession: NM_080678, NP_542409

UniProt: Q969M7