GCLM antibody (Middle Region)

Catalog No. ABIN2785805

This Rabbit Polyclonal antibody specifically detects GCLM in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse and Zebrafish (Danio rerio) and has been mentioned in 2+ publications.

Quick Overview for GCLM antibody (Middle Region) (ABIN2785805)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GCLM Antibody

Binding Specificity: Middle Region

Sequence: KPNSNQVNLA SCCVMPPDLT AFAKQFDIQL LTHNDPKELL SEASFQEALQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against GCLM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GCLM

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 274 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GCLM antibody (ABIN2785805)

Tomasi, Ryoo, Yang, Iglesias Ara, Ko, Lu: "Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice." in: Free radical biology & medicine, Vol. 68, pp. 148-58, (2014) (PubMed).

Ramani, Tomasi, Yang, Ko, Lu: "Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 36341-55, (2012) (PubMed).

Target Details for GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Alternative Name: GCLM

Background: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: GLCLR
Protein Interaction Partner: UBC, NAGK, GLRX3, GNAI2, GCLC, STOX1, IRF7, CALM1, SORD, CUL3,
Protein Size: 274

Molecular Weight: 31 kDa

Gene ID: 2730

NCBI Accession: NM_002061, NP_002052

UniProt: P48507