ORC6 antibody (C-Term)

Catalog No. ABIN2786008

Product Details anti-ORC6 Antibody

Target: ORC6 (Origin Recognition Complex, Subunit 6 (ORC6))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORC6 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VEAPAKEMEK VEEMPHKPQK DEDLTQDYEE WKRKILENAA SAQKATAE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against ORC6L. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ORC6L

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 252 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ORC6

Target: ORC6 (Origin Recognition Complex, Subunit 6 (ORC6))

Alternative Name: ORC6L (ORC6 Products)

Background: The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. ORC6L is a subunit of the ORC complex. It has been shown that this protein and and ORC1L are loosely associated with the core complex consisting of ORC2L, -3L, -4L and -5L. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis.The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown that this protein and and ORC1L are loosely associated with the core complex consisting of ORC2L, -3L, -4L and -5L. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ORC6, ORC6L
Protein Interaction Partner: LHX4, SPAG5, UBC, ORC3, CDC7, ORC4, MCM7, MCM6, MCM4, MCM2, CDC6, APP, ORC2, SUMO2, XRCC5, XRCC6, HMGA1, MCM5, TERF2, ORC5, DBF4, CDC45, RPA1,
Protein Size: 252

Molecular Weight: 28 kDa

Gene ID: 23594

NCBI Accession: NM_014321, NP_055136

UniProt: Q9Y5N6

Pathways: Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA