DAAM1 antibody (Middle Region)

Catalog No. ABIN2786070

The Rabbit Polyclonal anti-DAAM1 antibody has been validated for WB. It is suitable to detect DAAM1 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Dog.

Quick Overview for DAAM1 antibody (Middle Region) (ABIN2786070)

Target: DAAM1 (Dishevelled Associated Activator of Morphogenesis 1 (DAAM1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAAM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DAAM1 Antibody

Binding Specificity: Middle Region

Sequence: GNTVQYWLLL DRIIQQIVIQ NDKGQDPDST PLENFNIKNV VRMLVNENEV

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against DAAM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DAAM1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1078 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DAAM1

Target: DAAM1 (Dishevelled Associated Activator of Morphogenesis 1 (DAAM1))

Alternative Name: DAAM1

Background: Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein.Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.
Alias Symbols: FLJ41657, KIAA0666
Protein Interaction Partner: UBC, PRPF40A, B3GALT4, AMBP, MAP3K7, DVL2, DVL1, RHOA,
Protein Size: 1078

Molecular Weight: 123 kDa

Gene ID: 23002

NCBI Accession: NM_014992, NP_055807

UniProt: Q9Y4D1

Pathways: WNT Signaling