SAV1 antibody (C-Term)

Catalog No. ABIN2787277

This Rabbit Polyclonal antibody specifically detects SAV1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Pig, Rabbit and Cow.

Quick Overview for SAV1 antibody (C-Term) (ABIN2787277)

Target: SAV1 (Salvador Homolog 1 (SAV1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAV1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SAV1 Antibody

Binding Specificity: C-Term

Sequence: PCAPSVPRYD QPPPITYQPQ QTERNQSLLV PANPYHTAEI PDWLQVYARA

Predicted Reactivity: Cow: 93%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Sav1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Sav1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 386 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SAV1

Target: SAV1 (Salvador Homolog 1 (SAV1))

Alternative Name: Sav1

Background: Sav1 is a regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation.
Alias Symbols: 1700040G09Rik, Sav, WW45, Wwp3, Wwp4
Protein Size: 386

Molecular Weight: 42 kDa

Gene ID: 64010

NCBI Accession: NM_022028, NP_071311

UniProt: Q8VEB2

Pathways: Stem Cell Maintenance