HMGB2 antibody (Middle Region)

Catalog No. ABIN2787574

The Rabbit Polyclonal anti-HMGB2 antibody has been validated for WB and IHC. It is suitable to detect HMGB2 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Pig and Zebrafish (Danio rerio).

Quick Overview for HMGB2 antibody (Middle Region) (ABIN2787574)

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HMGB2 Antibody

Binding Specificity: Middle Region

Sequence: DREMKNYVPP KGDKKGKKKD PNAPKRPPSA FFLFCSEHRP KIKSEHPGLS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 87%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 80%

Characteristics: This is a rabbit polyclonal antibody against HMGB2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HMGB2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 209 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HMGB2

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Alternative Name: HMGB2

Background: HMGB2 is a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. HMGB2 was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: HMG2
Protein Interaction Partner: PKNOX1, UBC, EED, YWHAG, PGD, LDHB, CKB, HMGA1, GZMK, GZMA, CSNK1A1, FN1, SCAF8, APP, TFAP4, CDK2, BKRF1, Cebpb, ELAVL1, HMGB1, CHAF1A, CREBBP, SET, PGR, POU3F1, PRKDC, TP53, RAG1, POU5F1, POU2F2, POU2F1, APEX1, AR, NR3C1,
Protein Size: 209

Molecular Weight: 24 kDa

Gene ID: 3148

NCBI Accession: NM_002129, NP_002120

UniProt: P26583

Pathways: Cellular Response to Molecule of Bacterial Origin