ATG7 antibody (C-Term)

Catalog No. ABIN2787928

This Rabbit Polyclonal antibody specifically detects ATG7 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig and Horse.

Quick Overview for ATG7 antibody (C-Term) (ABIN2787928)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATG7 Antibody

Binding Specificity: C-Term

Sequence: KLVINAALGF DTFVVMRHGL KKPKQQGAGD LCPSHLVAPA DLGSSLFANI

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Atg7. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Rat Atg7

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 698 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: Atg7

Background: Atg7 functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. It forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. It also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3.
Alias Symbols: Apg7l
Protein Size: 698

Molecular Weight: 76 kDa

Gene ID: 312647

NCBI Accession: NM_001012097, NP_001012097

UniProt: Q641Y5

Pathways: Response to Water Deprivation, Autophagy