Factor VIII antibody (C-Term)

Catalog No. ABIN2788318

The Rabbit Polyclonal anti-Factor VIII antibody has been validated for WB. It is suitable to detect Factor VIII in samples from Human, Dog, Rabbit, Cow, Sheep and Horse.

Quick Overview for Factor VIII antibody (C-Term) (ABIN2788318)

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Reactivity: Human, Dog, Rabbit, Cow, Sheep, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Factor VIII antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Factor VIII Antibody

Binding Specificity: C-Term

Sequence: IMVTFRNQAS RPYSFYSSLI SYEEDQRQGA EPRKNFVKPN ETKTYFWKVQ

Predicted Reactivity: Cow: 86%, Dog: 77%, Horse: 93%, Human: 100%, Rabbit: 86%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against F8. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human F8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 2351 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Factor VIII

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Alternative Name: F8

Background: This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation, factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.
Alias Symbols: AHF, DXS1253E, F8B, F8C, FVIII, HEMA
Protein Interaction Partner: UBQLN1, GGA1, MCFD2, PHYH, LMAN1, F9, F10, VWF, F2, PROS1, PROC, HSPA5, CANX, CALR, ASGR2, LRP1,
Protein Size: 2351

Molecular Weight: 79 kDa

Gene ID: 2157

NCBI Accession: NM_000132, NP_000123

UniProt: P00451