PYM antibody (Middle Region)

Catalog No. ABIN2788484

Product Details anti-PYM Antibody

Target: PYM (WIBG) (Partner of Y14 and mago (WIBG))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Cow, Dog, Horse, Guinea Pig, Rabbit, Rat, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYM antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GLSKTAKRNL KRKEKRRQQQ EKEAEALSRT LDKVSLGDTA QIPSALQGPQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Yeast: 82%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against Wibg. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Mouse Wibg

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PYM

Target: PYM (WIBG) (Partner of Y14 and mago (WIBG))

Alternative Name: Wibg (WIBG Products)

Background: Wibg is a key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. It acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Wibg interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions.
Alias Symbols: A030010B05Rik, Pym
Protein Size: 202

Molecular Weight: 22 kDa

Gene ID: 78428

NCBI Accession: NM_001170869, NP_001164340