14-3-3 theta antibody (N-Term)

Catalog No. ABIN2792144

The Rabbit Polyclonal anti-14-3-3 theta antibody has been validated for WB and IHC. It is suitable to detect 14-3-3 theta in samples from Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for 14-3-3 theta antibody (N-Term) (ABIN2792144)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-14-3-3 theta Antibody

Binding Specificity: N-Term

Sequence: SVAYKNVVGG RRSAWRVISS IEQKTDTSDK KLQLIKDYRE KVESELRSIC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against YWHAQ. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human YWHAQ

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 245 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: YWHAQ

Background: YWHAQ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
Alias Symbols: 1C5, HS1, 14-3-3
Protein Interaction Partner: UBC, TNFAIP3, TRIM42, RAF1, PIK3R2, PIK3C3, FUS, DTL, MAPT, ABL1, SGK1, SIK3, SUMO2, TUBGCP3, IRS4, HDAC5, HDAC4, STAU1, MDM2, TGM2, SMS, ALDH7A1, YWHAZ, YWHAH, GNAI3, G6PD, EIF5, EEF2, ECHS1, CAPNS1, CAPN1, LDHB, YWHAG, YWHAB, VCL, UBE2V1, TXNDC5, SMEK2,
Protein Size: 245

Molecular Weight: 28 kDa

Gene ID: 10971

NCBI Accession: NM_006826, NP_006817

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction