PRDM1 antibody (Middle Region)

Catalog No. ABIN2792524

This anti-PRDM1 antibody is a Rabbit Polyclonal antibody detecting PRDM1 in WB and IHC. Suitable for Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit and Guinea Pig. This Primary Antibody has been cited in 1 publication.

Quick Overview for PRDM1 antibody (Middle Region) (ABIN2792524)

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRDM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PRDM1 Antibody

Binding Specificity: Middle Region

Sequence: VEDDISVISV VEKEILAVVR KEKEETGLKV SLQRNMGNGL LSSGCSLYES

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 84%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against PRDM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PRDM1

Application Details

Application Notes: WB Suggested Anti-PRDM1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: SH-SYSY cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 691 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PRDM1 antibody (ABIN2792524)

Arulanandam, Batenchuk, Angarita, Ottolino-Perry, Cousineau, Mottashed, Burgess, Falls, De Silva, Tsang, Howe, Bourgeois-Daigneault, Conrad, Daneshmand, Breitbach, Kirn, Raptis, Sad, Atkins, Huh et al.: "VEGF-Mediated Induction of PRD1-BF1/Blimp1 Expression Sensitizes Tumor Vasculature to Oncolytic Virus Infection. ..." in: Cancer cell, Vol. 28, Issue 2, pp. 210-24, (2015) (PubMed).

Target Details for PRDM1

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Alternative Name: PRDM1 / BLIMP1

Background: PRDM1 is a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.
Alias Symbols: BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1
Protein Interaction Partner: PRMT5, SENP1, EHMT2, PIAS1, SUMO1, IRF2, IRF1, HDAC2, HDAC1, HIST1H1A, MS4A1, HSP90AA1, ATXN1, ELAVL1, KDM1A, IRF4, TLE2, TLE1, AES, PRMT7, HIST2H3A,
Protein Size: 691

Molecular Weight: 77 kDa

Gene ID: 639

NCBI Accession: NM_182907, NP_878911

UniProt: Q86WM7

Pathways: Regulation of Muscle Cell Differentiation