NR2F2 antibody (N-Term)

Catalog No. ABIN2792599

This Rabbit Polyclonal antibody specifically detects NR2F2 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Zebrafish (Danio rerio) and Sheepand has been mentioned in 1 publication.

Quick Overview for NR2F2 antibody (N-Term) (ABIN2792599)

Target: NR2F2 (Nuclear Receptor Subfamily 2, Group F, Member 2 (NR2F2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Zebrafish (Danio rerio), Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR2F2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NR2F2 Antibody

Binding Specificity: N-Term

Sequence: KVGMRREAVQ RGRMPPTQPT HGQFALTNGD PLNCHSYLSG YISLLLRAEP

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Predicted Reactivity: Cow: 92%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 92%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against NR2F2. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NR2F2

Application Details

Application Notes: WB Suggested Anti-NR2F2 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 414 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NR2F2 antibody (ABIN2792599)

Déjardin, Kingston: "Purification of proteins associated with specific genomic Loci." in: Cell, Vol. 136, Issue 1, pp. 175-86, (2009) (PubMed).

Target Details for NR2F2

Target: NR2F2 (Nuclear Receptor Subfamily 2, Group F, Member 2 (NR2F2))

Alternative Name: NR2F2

Background: NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing transcription. However, in certain contexts, NR2F2 stimulates transcription directly. NR2F2, MyoD and p300 interact in a competitive manner, and that increasing amounts of NR2F2 have the ability to reduce the interaction between myoD and p300 invitro. NR2F2 post-transcriptionally regulates myoD activity/function, and that crosstalk between orphan nuclear receptors and the myogenic bHLH proteins has functional consequences for differentiation.
Alias Symbols: ARP1, COUP-TFII, COUPTFB, MGC117452, SVP40, TFCOUP2, NF-E3, NR2F1, COUPTFII
Protein Interaction Partner: NSD1, BCL11A, NR2F2, BIK, SETD7, FAM46A, HIPK3, UBC, TFAP4, Cebpb, SMARCAD1, NR2F6, POU5F1, NR3C1, PHB2, TRIP4, PIAS1, BCL11B, TRIM24, EP300, SQSTM1, HDAC1, NCOR2, MYOD1, LCK,
Protein Size: 414

Molecular Weight: 45 kDa

Gene ID: 7026

NCBI Accession: NM_021005, NP_066285

UniProt: P24468

Pathways: Steroid Hormone Mediated Signaling Pathway