IRF8 antibody (N-Term)

Catalog No. ABIN184318

This Rabbit Polyclonal antibody specifically detects IRF8 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for IRF8 antibody (N-Term) (ABIN184318)

Target: IRF8 (Interferon Regulatory Factor 8 (IRF8))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IRF8 Antibody

Binding Specificity: N-Term

Sequence: MFRIPWKHAG KQDYNQEVDA SIFKAWAVFK GKFKEGDKAE PATWKTRLRC

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against IRF8. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human IRF8

Application Details

Application Notes: WB Suggested Anti-IRF8 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 426 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for IRF8

Target: IRF8 (Interferon Regulatory Factor 8 (IRF8))

Alternative Name: IRF8 / ICSBP1

Background: Interferon regulatory factor 8 (IRF8, interferon consensus sequence-binding protein, Ion ChannelSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-. and IFN-.. IRF family proteins also control expression of IFN-. and IFN-.-regulated genes that are induced by viral infection.
Alias Symbols: ICSBP, IRF-8, ICSBP1, H-ICSBP
Protein Interaction Partner: SPI1, IRF1, TOP1, ZBTB17, PDGFRB, CBL, UBC, COPS4, COPS5, COPS6, COPS2, MID1, IRF6, GPS1, CDK4, SQSTM1, TRAF6, SMURF2, IRF2, TRIM21, IRF8, ETV6, RELA, NFKB1,
Protein Size: 426

Molecular Weight: 48 kDa

Gene ID: 3394

NCBI Accession: NM_002163, NP_002154

UniProt: Q02556

Pathways: Cellular Response to Molecule of Bacterial Origin