NHLH1 antibody (N-Term)

Catalog No. ABIN2792658

Product Details anti-NHLH1 Antibody

Target: NHLH1 (Nescient Helix Loop Helix 1 (NHLH1))

Binding Specificity: N-Term

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NHLH1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GGPGGGQARG PEPGEPGRKD LQHLSREERR RRRRATAKYR TAHATRERIR

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human

Predicted Reactivity: Cow: 92%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against NHLH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NHLH1

Application Details

Application Notes: WB Suggested Anti-NHLH1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: Human heart.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 133 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NHLH1

Target: NHLH1 (Nescient Helix Loop Helix 1 (NHLH1))

Alternative Name: HEN1 (NHLH1 Products)

Background: The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. Four members of this family have been clearly implicated in tumorigenesis via their involvement in chromosomal translocations in lymphoid tumors: MYC, LYL1, E2A, and SCLThe helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. Four members of this family have been clearly implicated in tumorigenesis via their involvement in chromosomal translocations in lymphoid tumors: MYC (MIM 190080), LYL1 (MIM 151440), E2A (MIM 147141), and SCL (MIM 187040).[supplied by OMIM]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments.
Alias Symbols: HEN1, NSCL, NSCL1, bHLHa35
Protein Interaction Partner: CENPP, PIM1, INO80E, PSME2, HIVEP1, ELAVL1, LMO4, CSRP3, LMO1, LMO2, xlmo1, TCF3,
Protein Size: 133

Molecular Weight: 14 kDa

Gene ID: 4807

NCBI Accession: NM_005598, NP_005589

UniProt: Q02575