TAF12 antibody (N-Term)

Catalog No. ABIN2792680

The Rabbit Polyclonal anti-TAF12 antibody has been validated for WB and ChIP. It is suitable to detect TAF12 in samples from Human, Rat, Mouse, Cow, Dog, Horse, Rabbit and Guinea Pig.

Quick Overview for TAF12 antibody (N-Term) (ABIN2792680)

Target: TAF12 (TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12))

Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAF12 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-TAF12 Antibody

Binding Specificity: N-Term

Sequence: MNQFGPSALI NLSNFSSIKP EPASTPPQGS MANSTAVVKI PGTPGAGGRL

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 85%, Rabbit: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against TAF12. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TAF12

Application Details

Application Notes: WB Suggested Anti-TAF12 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 161 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TAF12

Target: TAF12 (TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12))

Alternative Name: TAF12

Background: Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I.Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: TAF2J, TAFII20
Protein Interaction Partner: PRKAR2B, SUMO1, UBE2I, TAF4B, KAT2B, HIST3H3, SUPT3H, TAF4, ERCC1, TAF10, ELAVL1, CPSF1, TAF3, TBP, TAF1A, TAF1C, ATF7, ATXN7, NFYC, NFYB, NFYA, TAF6, TAF8, TAF9, TAF11, TAF5, TAF15, TAF7, TAF2, TAF1, MYC,
Protein Size: 161

Molecular Weight: 18 kDa

Gene ID: 6883

NCBI Accession: NM_005644, NP_005635

UniProt: Q16514