TBP antibody (Middle Region)

Catalog No. ABIN184358

The Rabbit Polyclonal anti-TBP antibody has been validated for WB. It is suitable to detect TBP in samples from Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Dog, Sheep and Guinea Pig. There is 1 publication available.

Quick Overview for TBP antibody (Middle Region) (ABIN184358)

Target: TBP (TATA Box Binding Protein (TBP))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Dog, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TBP Antibody

Binding Specificity: Middle Region

Sequence: FSSGKMVCTG AKSEEQSRLA ARKYARVVQK LGFPAKFLDF KIQNMVGSCD

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against TBP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TBP

Application Details

Application Notes: WB Suggested Anti-TBP Antibody Titration: 2.5 µg/mL
Positive Control: Human Placenta.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 339 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TBP antibody (ABIN184358)

Reid, van Roon-Mom, Wood, Rees, Owen, Faull, Dragunow, Snell: "TBP, a polyglutamine tract containing protein, accumulates in Alzheimer's disease." in: Brain research. Molecular brain research, Vol. 125, Issue 1-2, pp. 120-8, (2004) (PubMed).

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TBP (TATA-box-binding protein)

Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.
Alias Symbols: HDL4, GTF2D, SCA17, TFIID, GTF2D1
Protein Interaction Partner: DR1, SSX2IP, GOLGA2, MEF2A, TAF8, GNG12, GNB2, UBC, UBE2I, TAF10, TAF5, HNF4A, E2F1, PAX5, TAF1A, TCEA1, RUVBL2, Abt1, GTF2A2, ICE2, tat, MED26, TBP, TAF6, TAF4, TAF1, SP1, TP53, SNAPC2, SNAPC1, MUC1, Taf1c, Taf1b, GTF2B, HCVgp1, AHR, TAF9B, BTAF1, TAF13,
Protein Size: 339

Molecular Weight: 38 kDa

Gene ID: 6908

NCBI Accession: NM_003194, NP_003185

UniProt: P20226

Pathways: WNT Signaling