GTF2B antibody (N-Term)

Catalog No. ABIN184361

The Rabbit Polyclonal anti-GTF2B antibody has been validated for WB and IHC. It is suitable to detect GTF2B in samples from Human, Mouse, Dog, Zebrafish (Danio rerio), Horse, Guinea Pig and Rabbit. There is 1 publication available.

Quick Overview for GTF2B antibody (N-Term) (ABIN184361)

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Reactivity: Human, Mouse, Dog, Zebrafish (Danio rerio), Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GTF2B Antibody

Binding Specificity: N-Term

Sequence: NLPRNIVDRT NNLFKQVYEQ KSLKGRANDA IASACLYIAC RQEGVPRTFK

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against GTF2B. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GTF2B

Application Details

Application Notes: WB Suggested Anti-GTF2B Antibody Titration: 4.0 µg/mL
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 316 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GTF2B antibody (ABIN184361)

Tubon, Tansey, Herr: "A nonconserved surface of the TFIIB zinc ribbon domain plays a direct role in RNA polymerase II recruitment." in: Molecular and cellular biology, Vol. 24, Issue 7, pp. 2863-74, (2004) (PubMed).

Target Details for GTF2B

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Alternative Name: GTF2B

Background: GTF2B is the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.
Alias Symbols: TF2B, TFIIB
Protein Interaction Partner: DRAP1, TNIP1, UBC, FBXO25, HNF4A, TCEA1, GTF2F2, GTF2F1, DEPDC7, SLC22A2, RELA, POLR2C, JAK3, HMGCL, SMURF1, THRA, TBP, POLR2E, SRA1, LSM4, LSM5, CIITA, NXF1, POU3F2, EP300, PSMA3, POLR2J, ESR1, UL48, NR1D2, NCOR1, TAF9, REST, tat, SAP25, MKX, CTDP1, POLR
Protein Size: 316

Molecular Weight: 35 kDa

Gene ID: 2959

NCBI Accession: NM_001514, NP_001505

UniProt: Q00403