MAP4K1 antibody (pSer171)

Catalog No. ABIN2794221

This anti-MAP4K1 antibody is a Rabbit Polyclonal antibody detecting MAP4K1 in DB. Suitable for Human.

Quick Overview for MAP4K1 antibody (pSer171) (ABIN2794221)

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K1 antibody is un-conjugated

Application: Dot Blot (DB)

Product Details anti-MAP4K1 Antibody

Binding Specificity: pSer171

Purpose: Rabbit Anti-Human Phospho-MAP4K1(S171) Antibody

Immunogen: This MAP4K1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S171 of human MAP4K1.

Isotype: Ig Fraction

Application Details

Application Notes: Dot Blot
Recommended Dilutions
DB: 1:500
Dot blot analysis of anti-MAP4K1-pS171 Phospho-specific Pab (RB12751) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 μg per ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.340 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for MAP4K1

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Alternative Name: MAP4K1

Background:

Target Description: The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.

Gene Symbol: MAP4K1

Molecular Weight: 91296 Da

Gene ID: 11184

UniProt: Q92918

Pathways: TCR Signaling, Signaling of Hepatocyte Growth Factor Receptor