WT1 antibody (AA 346-375)

Catalog No. ABIN2797552

This Rabbit Polyclonal antibody specifically detects WT1 in IHC, IF, FACS and WB. It exhibits reactivity toward Human.

Quick Overview for WT1 antibody (AA 346-375) (ABIN2797552)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)

Product Details anti-WT1 Antibody

Binding Specificity: AA 346-375

Purpose: Rabbit Anti-Human WT1 (Center E361) Antibody

Immunogen: This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human WT1.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Recommended Dilutions
WB: 1:1000, IHC: 1:50-100, IF: 1:10-50, FC: 1:10-50WT1 Antibody (Center E361) .FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.300 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background:

Target Description: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].

Gene Symbol: WT1

Molecular Weight: 49188 Da

Gene ID: 7490

UniProt: P19544

Pathways: Tube Formation