LIN28B antibody (C-Term)

Catalog No. ABIN2798788

This Rabbit Polyclonal antibody specifically detects LIN28B in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for LIN28B antibody (C-Term) (ABIN2798788)

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN28B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-LIN28B Antibody

Binding Specificity: AA 218-250, C-Term

Purpose: Rabbit Anti-Human LIN28B (C-term) Antibody

Immunogen: This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-250 amino acids from the C-terminal region of human LIN28B.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Flow Cytometry
Recommended Dilutions
WB: 1:1000, FC: 1:10-50Western blot analysis of LIN28B Antibody (C-term) in HL60 cell line lysates (35 μg/lane). LIN28B (arrow) was detected using the purified Pab.Flow cytometric analysis of HL-60 cells using LIN28B Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for LIN28B

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Alternative Name: LIN28B

Background:

Target Description: Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.

Gene Symbol: LIN28B

Molecular Weight: 27084 Da

Gene ID: 389421

UniProt: Q6ZN17