DKK3 antibody (N-Term)

Catalog No. ABIN2798864

The Rabbit Polyclonal anti-DKK3 antibody has been validated for WB and IHC. It is suitable to detect DKK3 in samples from Human.

Quick Overview for DKK3 antibody (N-Term) (ABIN2798864)

Target: DKK3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DKK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DKK3 Antibody

Binding Specificity: AA 15-45, N-Term

Purpose: Rabbit Anti-Human DKK3 (N-term) Antibody

Immunogen: This DKK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human DKK3.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:50-100Western blot analysis of lysates from 293, 293T, A549, HL-60, MCF-7 cell line and mouse brain tissue lysate (from left to right), using DKK3 Antibody (A30), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.500 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for DKK3

Target: DKK3

Background:

Target Description: DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

Gene Symbol: DKK3

Molecular Weight: 38390 Da

Gene ID: 27122

UniProt: Q9UBP4