Recoverin antibody (C-Term)

Catalog No. ABIN2798878

This Rabbit Polyclonal antibody specifically detects Recoverin in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Recoverin antibody (C-Term) (ABIN2798878)

Target: Recoverin (RCVRN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Recoverin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Recoverin Antibody

Binding Specificity: AA 169-200, C-Term

Purpose: Rabbit Anti-Human Recoverin (C-term) Antibody

Immunogen: This Recoverin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-200 amino acids from the C-terminal region of human Recoverin.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:50-100The anti-Recoverin C-term Pab is used in Western blot to detect Recoverin in Y79 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated. BC = breast carcinoma, HC = hepatocarcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for Recoverin

Target: Recoverin (RCVRN)

Alternative Name: Recoverin

Background:

Target Description: Recoverin belongs to a high-affinity calcium-binding family that includes neuronal calcium sensor-1, visinin-like proteins (VILIPs), guanylate cyclase-activating proteins (GCAPs), and Kv-channel interacting proteins (KchIPs). Features common to this family include four calcium-binding EF-hand domains, and an N-terminal myristoylation sequence. This family of proteins has been implicated in a broad range of cellular signaling functions, including phototransduction and neurotransmitter release, lipid metabolism, gene expression, and ion channel regulation. Myristoylation, the post-translational addition of a fatty acid tail, has been shown to have functional significance for other calcium-binding protein family members. Recoverin is subject to the posttranslational modification of myristoylation. Binding of calcium to recoverin elicits a change in conformation that exposes the buried hydrophobic myristoyl moiety to interaction with cell membranes and other cellular proteins.

Gene Symbol: RCVRN

Molecular Weight: 23130 Da

Gene ID: 5957

UniProt: P35243

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction