ERAS antibody (N-Term)

Catalog No. ABIN2800622

The Rabbit Polyclonal anti-ERAS antibody has been validated for WB, IHC and IF. It is suitable to detect ERAS in samples from Human.

Quick Overview for ERAS antibody (N-Term) (ABIN2800622)

Target: ERAS (ES cell expressed Ras (ERAS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: F66

Product Details anti-ERAS Antibody

Binding Specificity: AA 51-81, N-Term

Purpose: Rabbit Anti-Human ERAS (N-term) (F66) Antibody

Immunogen: This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-81 amino acids from the N-terminal region of human ERAS.

Isotype: Ig Fraction

Application Details

Application Notes: Immunofluorescence, Western Blot, Immunohistochemistry
Recommended Dilutions
IF: 1:100, WB: 1:1000, IHC: 1:10-50Fluorescent confocal image of SY5Y cells stained with ERAS (N-term) (F66) antibody. SY5Y cells were fixed with 4 % PFA (20 min), permeabilized with Triton X-100 (0.2 %, 30 min). Cells were then incubated with 102-12613 ERAS (N-term) (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/mL, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.Western blot analysis of ERAS Antibody (N-term) (F66) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for ERAS

Target: ERAS (ES cell expressed Ras (ERAS))

Alternative Name: ERAS

Background:

Target Description: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.

Gene Symbol: ERAS

Molecular Weight: 25287 Da

Gene ID: 3266

UniProt: Q7Z444