GABARAP antibody (N-Term)

Catalog No. ABIN2800631

The Rabbit Polyclonal anti-GABARAP antibody has been validated for WB, IHC and IF. It is suitable to detect GABARAP in samples from Human.

Quick Overview for GABARAP antibody (N-Term) (ABIN2800631)

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABARAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GABARAP Antibody

Binding Specificity: AA 1-30, N-Term

Purpose: Rabbit Anti-Human GABARAP (N-term) Antibody

Immunogen: This GABARAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GABARAP.

Isotype: Ig Fraction

Application Details

Application Notes: Immunofluorescence, Western Blot, Immunohistochemistry
Recommended Dilutions
IF: 1:10-50, WB: 1:1000, IHC: 1:10-50Fluorescent image of U251 cells stained with GABARAP (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4 % PFA (20 min), permeabilized with Triton X-100 (0.2 %, 30 min). Cells were then incubated with 102-13733 GABARAP (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/mL, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Western blot analysis of GABARAP (arrow) using purified Pab .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Alternative Name: GABARAP

Background:

Target Description: Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABARAP is GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

Gene Symbol: GABARAP

Molecular Weight: 13918 Da

Gene ID: 11337

UniProt: O95166

Pathways: Autophagy